S5a/Angiocidin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-3318

Novus Biologicals, part of Bio-Techne
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NB120-3318
NB120-3318-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all S5a/Angiocidin Antibodies »

Product Summary for S5a/Angiocidin Antibody

Immunogen

Human recombinant proteasome 19S subunit S5A.

Localization

Nuclear

Specificity

Detects proteasome 19S subunit S5A.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for S5a/Angiocidin Antibody

Western Blot: S5a/Angiocidin Antibody [NB120-3318]

Western Blot: S5a/Angiocidin Antibody [NB120-3318]

Western Blot: Proteasome 19S S5A Antibody [NB120-3318] - Analysis of HeLa cell lysate.

Applications for S5a/Angiocidin Antibody

Application
Recommended Usage

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500
Application Notes
WB: Detects an approx. 50 kDa protein representing proteasome 19S subunit S5A in HeLa cell lysate.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: S5a/Angiocidin

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

Long Name

26S Proteasome Regulatory Subunit S5a

Alternate Names

Angiocidin, ASF, Macropain, Mcb1, PSMD4, pUB-R5, Rpn10, S5a

Entrez Gene IDs

5710 (Human)

Gene Symbol

PSMD4

UniProt

P55036 (Human)

Additional S5a/Angiocidin Products

Product Documents for S5a/Angiocidin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for S5a/Angiocidin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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