Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all SA1 Antibodies »

Product Specifications

Immunogen

A 35 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 1 encoded in part by exons 35 and 36 (LocusLink ID 10274).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SA1 Antibody

Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody NB100-299 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/ Immunofluorescence: SA1 Antibody [NB100-299]

Immunocytochemistry/ Immunofluorescence: SA1 Antibody [NB100-299]

Immunocytochemistry/Immunofluorescence: SA1 Antibody [NB100-299] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.
Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.
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Applications for SA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2000

Western Blot

1:1000-1:10000
Application Notes
NB 100-299 may be used for Western Blot where a band at ~145 kD is seen on SDS-PAGE. It may also be used for immunocytochemistry. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SA1

SA1 is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Alternate Names

cohesin subunit SA-1, nuclear protein stromal antigen 1, SA1, stromal antigen 1

Gene Symbol

STAG1

Additional SA1 Products

Product Documents for SA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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