SAFB Antibody (6F7)
Novus Biologicals, part of Bio-Techne | Catalog # NB200-332
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6F7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human SAFB protein.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This is specific for HET SAF B protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SAFB Antibody (6F7)
![Western Blot: SAFB Antibody (6F7) [NB200-332]](https://resources.bio-techne.com/images/products/SAFB-Antibody-6F7-Western-Blot-NB200-332-img0004.jpg "Western Blot: SAFB Antibody (6F7) [NB200-332]")
Western Blot: SAFB Antibody (6F7) [NB200-332]
Western Blot: SAFB Antibody (6F7) [NB200-332] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5% SDS-PAGE SAFB antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: SAFB Antibody (6F7) [NB200-332]](https://resources.bio-techne.com/images/products/SAFB-Antibody-6F7-Immunocytochemistry-Immunofluorescence-NB200-332-img0002.jpg "Immunocytochemistry/ Immunofluorescence: SAFB Antibody (6F7) [NB200-332]")
Immunocytochemistry/ Immunofluorescence: SAFB Antibody (6F7) [NB200-332]
Immunocytochemistry/Immunofluorescence: SAFB Antibody (6F7) [NB200-332] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, antibody at 1:200 dilution.Applications for SAFB Antibody (6F7)
Application
Recommended Usage
Western Blot
1-2 ug/ml
Application Notes
ICC/IF, IHC, IP- Assay dependent
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SAFB
Alternate Names
glutathione S-transferase fusion protein, HAP, heat-shock protein (HSP27) estrogen response element and TATA box-bindingprotein, HETDKFZp779C1727, Hsp27 ERE-TATA binding protein, HSP27 ERE-TATA-binding protein, HSP27 estrogen response element-TATA box-binding protein, SAF-B, SAF-B1, SAFB1SAB-B1, scaffold attachment factor B, scaffold attachment factor B1
Gene Symbol
SAFB
Additional SAFB Products
Product Documents for SAFB Antibody (6F7)
Product Specific Notices for SAFB Antibody (6F7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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