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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7F5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-250 of human SAMSN1(NP_071419) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAMSN1 Antibody (OTI7F5) - Azide and BSA Free

Western Blot: SAMSN1 Antibody (OTI7F5)Azide and BSA Free [NBP2-73984]

Western Blot: SAMSN1 Antibody (OTI7F5)Azide and BSA Free [NBP2-73984]

Western Blot: SAMSN1 Antibody (OTI7F5) - Azide and BSA Free [NBP2-73984] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMSN1 .
Immunohistochemistry: SAMSN1 Antibody (OTI7F5) - Azide and BSA Free [NBP2-73984]

Immunohistochemistry: SAMSN1 Antibody (OTI7F5) - Azide and BSA Free [NBP2-73984]

Immunohistochemistry: SAMSN1 Antibody (OTI7F5) - Azide and BSA Free [NBP2-73984] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: SAMSN1 Antibody (OTI7F5) - Azide and BSA Free [NBP2-73984]

Immunohistochemistry: SAMSN1 Antibody (OTI7F5) - Azide and BSA Free [NBP2-73984]

Immunohistochemistry: SAMSN1 Antibody (OTI7F5) - Azide and BSA Free [NBP2-73984] - Analysis of Human tonsil tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Applications for SAMSN1 Antibody (OTI7F5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SAMSN1

SAMSN1 is a member of a novel gene family of putative adaptors and scaffold proteins containing SH3 and SAM (sterile alpha motif) domains. It also contains several predicted consensus nuclear localization signals and a tyrosine kinase phosphorylation motif. Northern blot analysis has revealed expression of a 2.2kb SAMSN1 transcript in human immune tissues and hematopoietic cell types including normal bone marrow, acute myeloid leukemia, and multiple myeloma; low-level expression was seen in other tissues such as heart, brain, placenta, and lung. SAMSN1 has been shown to be up-regulated by B cell activation signals and is a participant in B cell activation and differentiation.

Alternate Names

HACS1gene with homology to KIAA0790 protein10NASH1, Hematopoietic adaptor containing SH3 and SAM domains 1, SAM and SH3 domain containing 1, SAM and SH3 domain containing 2, SAM domain, SH3 domain and nuclear localisation signals, 1, SAM domain, SH3 domain and nuclear localization signals 1, SAM domain, SH3 domain and nuclear localization signals protein 1, SAM domain-containing protein SAMSN-1, SH3D6B, SH3-SAM adaptor protein

Gene Symbol

SAMSN1

Additional SAMSN1 Products

Product Documents for SAMSN1 Antibody (OTI7F5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SAMSN1 Antibody (OTI7F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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