SAP30L Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-491

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SAP30L Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to residues 165-183 (VKSNKSRLDQKSEGGKQLE) of human SAP30L.

Reactivity Notes

This antibody reacts with wild-type transfected and endogenous human SAP30L protein. This antibody does not react with endogenous non-human SAP30L.

Specificity

This is specific for SAP30L.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAP30L Antibody - BSA Free

Western Blot: SAP30L AntibodyBSA Free [NB100-491]

Western Blot: SAP30L AntibodyBSA Free [NB100-491]

Western Blot: SAP30L Antibody [NB100-491] - Analysis of SAP30L using NB100-491 on GST-hSAP30L fusion protein in NIH3T3 cells (Lane 1), wild-type transfected hSAP30L and endogenous hSAP30L in HEK293 cells, (Lane 2), and endogenous hSAP30L in non-transfected MCF-7 cells.

Applications for SAP30L Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
This antibody has been tested in Western blot. No other applications have been tested. Recommended dilution below. Western Blot - 1:500 to 1:1,000* *However, the investigator should determine the optimal dilution. The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kDa and the endogenous hSAP30L band runs with an apparent molecular weight of 21-22 kDa. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be to cleavage product, degradation and/or post-translational modification.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAP30L

A novel TGF- beta up-regulated mRNA species, the Sin3-associated protein 30-like, SAP30L has been identified. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. By reason of its nuclear localization and close homology to SAP30 it is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co-repressor complexes in response to TGF-beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells.

Alternate Names

DKFZp667L2214, FLJ11526, FLJ36497, HCV non-structural protein 4A-transactivated protein 2, histone deacetylase complex subunit SAP30L, NS4ATP2FLJ23595, SAP30-like, Sin3 corepressor complex subunit SAP30L, Sin3A associated protein p30-like, Sin3-associated protein p30-like

Gene Symbol

SAP30L

Additional SAP30L Products

Product Documents for SAP30L Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SAP30L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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