SAR1B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06074

Novus Biologicals, part of Bio-Techne
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NBP1-06074
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SAR1B Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DHERLLESKEELD corresponding to internal region according to NP_057187.1.

Epitope

C-DHERLLESKEELD

Specificity

Both reported variants (NP_001028675.1 and NP_057187.1) represent identical protein

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SAR1B Antibody

Western Blot: SAR1B Antibody [NBP1-06074]

Western Blot: SAR1B Antibody [NBP1-06074]

Western Blot: SAR1B Antibody [NBP1-06074] - (0.03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: SAR1B Antibody [NBP1-06074]

Western Blot: SAR1B Antibody [NBP1-06074]

Western Blot: SAR1B Antibody [NBP1-06074] - HEK293 overexpressing SAR1B and probed with (mock transfection in first lane).

Applications for SAR1B Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in mouse liver and rat liver lysates (calculated MW of 22.4 kDa band according to human NP_057187.1 and mouse NP_079811.1). In transfected HEK293 transiently expressing SAR1B a band of approx. 26 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SAR1B

SAR1B belongs to the small GTPase superfamily, SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus and is activated by the guanine nucleotide exchange factor PREB. SAR1B is involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The conditions are characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets.

Alternate Names

2310075M17Rik, ANDD, CMRD, EC 3.6.5, GTP-binding protein B, GTP-binding protein SAR1b, GTP-binding protein Sara, SAR1 homolog B (S. cerevisiae), SAR1a gene homolog (S. cerevisiae) 2, SAR1a gene homolog 2, SAR1a gene homolog 2 (S. cerevisiae), SARA2GTBPB, SARB

Entrez Gene IDs

51128 (Human); 66397 (Mouse); 287276 (Rat)

Gene Symbol

SAR1B

UniProt

NP_001028675.1 (Human)

Additional SAR1B Products

Product Documents for SAR1B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SAR1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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