SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25646

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25646
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Key Product Details

Species Reactivity

Validated:

SARS-CoV-2

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1443

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SARS-CoV-2 Envelope Antibodies »

Product Summary for SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of SARS-CoV-2 Envelopee. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free

SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free [NBP3-25646] -

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free [NBP3-25646] - SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] detects SARS-CoV-2 (COVID-19) Envelope protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Envelope stained by SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] (NBP3-25646) diluted at 1:500. Blue: Fluoroshield with DAPI .
SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free

Western Blot: SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free [NBP3-25646] -

Western Blot: SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free [NBP3-25646] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] (NBP3-25646) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 Envelope

The SARS-CoV-2 Envelope protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). The envelope protein is the smallest of the four structural proteins, which also includes the membrane protein, spike protein, and nucleocapsid protein (1,2). The envelope protein is synthesized as a 75 amino acid protein with a theoretical molecular weight of approximately 8.4 kDa (2,3). Furthermore, the envelope protein of SARS-CoV-2 has 94.7% sequence identity and 97.4% sequence similarity to the envelope protein of SARS-CoV (2). Structurally, the envelope protein is a membrane protein with a N-terminal domain, an alpha-helical transmembrane domain, and a hydrophilic C-terminal domain (1,4). The envelope protein has multiple functions in viral replication including viral assembly, release, and pathogenesis (2,4). Additionally, the SARS-CoV-2 envelope protein has ion channel activity and functions as a viroporin with a role in virion trafficking (2,4). Coronaviruses lacking the envelope protein are shown to have reduced viral titer and slowed or defective maturation, indicative of a role in virus production and growth (4).

References

1. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

2. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

3. Uniprot (P0DTC4)

4. J Alsaadi, E. A., & Jones, I. M. (2019). Membrane binding proteins of coronaviruses. Future Virology. https://doi.org/10.2217/fvl-2018-0144

Alternate Names

2019-nCoV Envelope protein, COVID-19 Envelope protein, E Protein, Envelope, Human Coronavirus Envelope protein, SARSCoV2, SARS-CoV-2 E protein, SARSCoV2 Envelope protein, SARS-CoV-2 Envelope protein, Severe acute respiratory syndrome 2 Envelope Protein

Gene Symbol

E

Additional SARS-CoV-2 Envelope Products

Product Documents for SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS-CoV-2 Envelope Antibody (HL1443) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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