SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25664
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
SARS-CoV-2
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1523
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 nsp8. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free [NBP3-25664] -
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free [NBP3-25664] - SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] detects SARS-CoV-2 (COVID-19) nsp8 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (NBP3-25664) diluted at 1:500. Blue: Fluoroshield with DAPI .
Immunohistochemistry-Paraffin: SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free [NBP3-25664] -
Immunohistochemistry-Paraffin: SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free [NBP3-25664] - SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] detects SARS-CoV-2 (COVID-19) nsp8 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mock and SARS-CoV-2 Nsp8 transfected 293T cell pellet. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (NBP3-25664) diluted at 1:100. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free [NBP3-25664] -
Western Blot: SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free [NBP3-25664] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (NBP3-25664) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 nsp8
References
1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4
2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9
3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39
4. Peng, Q., Peng, R., Yuan, B., Zhao, J., Wang, M., Wang, X., Wang, Q., Sun, Y., Fan, Z., Qi, J., Gao, G. F., & Shi, Y. (2020). Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2. Cell reports. https://doi.org/10.1016/j.celrep.2020.107774
Alternate Names
Non-structural protein 8, nsp8 replicase
Gene Symbol
ORF1ab
Additional SARS-CoV-2 nsp8 Products
Product Documents for SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free
Product Specific Notices for SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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