SARS-CoV-2 nsp8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13472

Novus Biologicals, part of Bio-Techne
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NBP3-13472
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SARS-CoV-2 nsp8 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 nsp8. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SARS-CoV-2 nsp8 Antibody

Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-13472]

Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-13472]

Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-13472] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13472) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 nsp8 Antibody [NBP3-13472]

Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 nsp8 Antibody [NBP3-13472]

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp8 Antibody [NBP3-13472] - SARS-CoV-2 (COVID-19) nsp8 antibody detects SARS-CoV-2 (COVID-19) nsp8 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13472) diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 nsp8 Antibody [NBP3-13472]

Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 nsp8 Antibody [NBP3-13472]

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp8 Antibody [NBP3-13472] - SARS-CoV-2 (COVID-19) nsp8 antibody detects SARS-CoV-2 (COVID-19) nsp8 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13472) diluted at 1:2000.
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Applications for SARS-CoV-2 nsp8 Antibody

Application
Recommended Usage

Western Blot

1:1000-1:10000
Application Notes
ICC/IF, IHC, IHC-P- Assay dependent.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp8

SARS-CoV-2 Nonstructural Protein 8 (NSP8) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP8 is 198 amino acids (aa) with a theoretical molecular weight of 21.9 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP8 has 97.5% sequence identity and 100% sequence similarity (1). SARS-CoV-2 NSP8 heterodimerizes with NSP7, which forms a complex with NSP12 (1-4). NSP8 alone in its monomeric form can also complex with NSP12, generating the RNA polymerase complex (1,4). Additionally, NSP8 is shown to interact with the SARS-CoV-2 open reading frame 6 (ORF6), encouraging RNA polymerase activity (1).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Peng, Q., Peng, R., Yuan, B., Zhao, J., Wang, M., Wang, X., Wang, Q., Sun, Y., Fan, Z., Qi, J., Gao, G. F., & Shi, Y. (2020). Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2. Cell reports. https://doi.org/10.1016/j.celrep.2020.107774

Alternate Names

Non-structural protein 8, nsp8 replicase

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp8 Products

Product Documents for SARS-CoV-2 nsp8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS-CoV-2 nsp8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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