Key Product Details

Species Reactivity

Validated:

SARS-CoV-2

Predicted:

SARS-CoV (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SARS-CoV-2 nsp9 Antibodies »

Product Specifications

Immunogen

This antibody was raised against a peptide corresponding to 13 amino acids near the center of SARS-CoV-2 (COVID-19) NSP9 protein. The immunogen is located within 30-80 amino acids of the SARS-CoV-2 (COVID-19) NSP9 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 nsp9 Antibody - BSA Free

Immunohistochemistry: SARS-CoV-2 nsp9 Antibody - BSA Free [NBP3-07044]

Immunohistochemistry: SARS-CoV-2 nsp9 Antibody - BSA Free [NBP3-07044]

Immunohistochemistry: SARS-CoV-2 nsp9 Antibody [NBP3-07044] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP9 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degrees celsius. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP9 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA: SARS-CoV-2 nsp9 Antibody - BSA Free [NBP3-07044]

ELISA: SARS-CoV-2 nsp9 Antibody - BSA Free [NBP3-07044]

ELISA: SARS-CoV-2 nsp9 Antibody [NBP3-07044] - Antbodies: SARS-CoV-2 (COVID-19) NSP9 Antibody. A direct ELISA was performed using immunogen peptide (9161P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP9 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.

Applications for SARS-CoV-2 nsp9 Antibody - BSA Free

Application
Recommended Usage

ELISA

1 ug/mL

Immunohistochemistry

0.5 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp9

SARS-CoV-2 Nonstructural Protein 9 (NSP9) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP9 is 113 amino acids (aa) with a theoretical molecular weight of 12.4 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP9 has 97.3% sequence identity and 99.1% sequence similarity (1). Structurally NSP9 has a N-terminal beta-barrel made of seven beta-strands and a C-terminal alpha-helix (3). In SARS-CoV, NSP9 is important for viral proliferation; it is unclear if NSP9 plays the same role in SARS-CoV-2 but given the high degree of sequence similarity it is quite likely (4). SARS-CoV-2 NSP9 also plays a role in formation of nuclear transport machinery and the extracellular matrix (2). Additionally, NSP9 binds mind bomb 1 (MIB1), an E3 ubiquitin ligase that regulates antiviral innate immune signaling (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Khan, M. T., Irfan, M., Ahsan, H., Ahmed, A., Kaushik, A. C., Khan, A. S., Chinnasamy, S., Ali, A., & Wei, D. Q. (2021). Structures of SARS-CoV-2 RNA-Binding Proteins and Therapeutic Targets. Intervirology. https://doi.org/10.1159/000513686

Alternate Names

Non-structural protein 9, nsp9 replicase

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp9 Products

Product Documents for SARS-CoV-2 nsp9 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS-CoV-2 nsp9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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