SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07043
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
SARS-CoV-2
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2A Clone # 1C7C7
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant nucleoprotein of SARS-CoV (severe acute respiratory syndrome).The nucleocapsid protein is expressed in the internal nucleocapsid of SARS-CoV-2.
Specificity
This antibody specifically targets an epitope on the SARS-CoV-2 and SARS-CoV nucleocapsid protein. This antibody does not cross-react with the nucleocapsid of other common human coronaviruses, OC43 and 229E
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free
N/A: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) [NBP3-07043] - Exp1293 cells were transfected with expression plasmid coding for SARS-CoV-2 Nucleocapsid and incubated for 48 hours. Cells were permeabilized, incubated with dilutions of supernatant collected from cells transfected with DNA plasmids coding for the heavy and light chains of 1C7 and binding identified by the addition of goat anti-mouse IgG FITC.
Western Blot: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free [NBP3-07043] -
Western Blot: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free [NBP3-07043] - Lysates were derived from A549 cells (Transduced with ACE2 by adenoviral vector), mock vs. SARS-CoV-2-infected MO12, 24 hours, with 60ug of lysates loaded/lane, Image Courtesy of Jeff Johnson Laboratory, ISMMS.
Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free [NBP3-07043] -
Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free [NBP3-07043] - Vero E6 infectedcwith SARS-CoV-2, Wa-1 @ MOI = 0.1 for 48 hours. Cells were fixed with 4 % formaldehyde (4hr), perm 0.1% Triton X (15min), blocked with 4 % BSA, 1:1000 diluted with clone 1C7C7 (Anti-SARS-CoV-2 Nucleocaspid) O/N tehn 1:1000 anti-mouse Dylight 488 (1 hour), Image Courtesy of Dr. Ana Fernandex - Sesma Laboratory, ISMMS.Applications for SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.01 M PBS (pH 7.2 - 7.4), 150 mM NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 Nucleocapsid
Following SARS-CoV-2 infection, B cells and T cells display an immune response against nucleocapsid protein and nucleocapsid-specific neutralizing antibodies are produced (5). Interestingly, a study of patients who have recovered from COVID-19 revealed that there were no neutralizing antibodies against nucleocapsid protein present; however, a high presence against neutralizing spike RBD antibodies were detected (5).
References
1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658
2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.
3. Zhu, G., Zhu, C., Zhu, Y., & Sun, F. (2020). Minireview of progress in the structural study of SARS-CoV-2 proteins. Current Research in Microbial Sciences. https://doi.org/10.1016/j.crmicr.2020.06.003
4. Uniprot (P0DTC9)
5. Shah, V. K., Firmal, P., Alam, A., Ganguly, D., & Chattopadhyay, S. (2020). Overview of Immune Response During SARS-CoV-2 Infection: Lessons From the Past. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01949
Alternate Names
2019-nCoV N Protein, 2019-nCoV Nucleocapsid Protein, COVID-19 N protein, COVID-19 nucleocapsid protein, Human coronavirus Nucleoprotein, nucleocapsid phosphoprotein, Nucleocapsid protein, Nucleoprotein, ORF9; structural protein, SARS-CoV-2, SARS-CoV-2 Nucleocapsid protein, SARS-CoV-2 N protein, SARSCoV2 Nucleoprotein, Severe Acute Respiratory Syndrome Coronavirus 2 Nucleocapsid Protein
Gene Symbol
N
Additional SARS-CoV-2 Nucleocapsid Products
Product Documents for SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free
Product Specific Notices for SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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