Key Product Details

Species Reactivity

SARS-CoV-2

Applications

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SARS-CoV-2 ORF10 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 13 amino acids near the carboxyl terminus of SARS-CoV-2 ORF10 protein. The immunogen is located within the last 50 amino acids of the SARS-CoV-2 ORF10 protein.

Specificity

Antibody is predicted to not cross-react with other coronavirus family members.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 ORF10 Antibody - BSA Free

ELISA: SARS-CoV-2 ORF10 Antibody - BSA Free [NBP3-11939]

ELISA: SARS-CoV-2 ORF10 Antibody - BSA Free [NBP3-11939]

ELISA: SARS-CoV-2 ORF10 Antibody [NBP3-11939] - ELISA Validation Antibodies: SARS-CoV-2 ORF10 Antibody, NBP3-11939. A direct ELISA was performed using SARS-CoV-2 ORF10 immunogen peptide as coating antigen and the anti-SARS-CoV-2 ORF10 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL

Applications for SARS-CoV-2 ORF10 Antibody - BSA Free

Application
Recommended Usage

ELISA

1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 ORF10

SARS-CoV-2 Open Reading Frame 10 (ORF10) is one of the nine downstream accessory protein open reading frames of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 ORF10 is the smallest accessory protein at 38 amino acids (aa) in length with a theoretical molecular weight of 4.4 kDa (1, 2). ORF10 is proposed to be unique to SARS-CoV-2, however it likely evolved from mutations in other coronaviruses (1, 3). Interestingly, the ORF10 of SARS-CoV-2 has 97.3% homology to Pangolin-CoV ORF10, but there are still prominent differences in properties such as protein structure, solubility, and mutational diversification (4).

Functionally, SARS-CoV-2 ORF10 is suggested to interact with the Cullin 2 RING E3 ligase complex, indicating that ORF10 might promote viral pathogenesis by taking over the ubiquitination machinery (4, 5). Additionally, the SARS-CoV-2 ORF10 gene appears to be differentially expressed based on COVID-19 severity. For example, ORF10 appears to be highly expressed in the infected cells from severe cases while it is either lowly expressed or not detectable in cells infected from moderate cases (6). Therefore, ORF10 expression could be indicative of COVID-19 progression (6).

References

1. Michel, C. J., Mayenr, C., Poch, O., & Thompson, J. D. (2020). Characterization of accessory genes in coronavirus genomes. Virology journal. https://doi.org/10.1186/s12985-020-01402-1

2. Uniprot (A0A6B9W2G5)

3. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The protein journal. https://doi.org/10.1007/s10930-020-09901-4

4. Hassan, S., Attrish, D., Ghosh, S., Choudhury, P., Uversky, V., Uhal, B., Lundstrom, K., Rezaei, N., Aljabali, A., Seyran, M., et al. (2020). Notable sequence homology of the ORF10 protein introspects the architecture of SARS-COV-2. bioRxiv. 10.1101/2020.09.06.284976.

5. Ahmadpour, D., & Ahmadpoor, P. (2020). How the COVID-19 Overcomes the Battle? An Approach to Virus Structure. Iranian journal of kidney diseases.

6. Liu, T., Jia, P., Fang, B., & Zhao, Z. (2020). Differential Expression of Viral Transcripts From Single-Cell RNA Sequencing of Moderate and Severe COVID-19 Patients and Its Implications for Case Severity. Frontiers in microbiology. https://doi.org/10.3389/fmicb.2020.603509

Alternate Names

2019-nCoV ORF10, 2019-nCoV ORF10 Protein, COVID-19 ORF10, Human coronavirus ORF10 Protein, ORF10, ORF10 protein, SARS-CoV-2, SARSCoV2 ORF10 Protein, SARS-CoV-2 ORF10 Protein, Severe Acute Respiratory Syndrome Coronavirus 2 ORF10 Protein

Gene Symbol

ORF10

Additional SARS-CoV-2 ORF10 Products

Product Documents for SARS-CoV-2 ORF10 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SARS-CoV-2 ORF10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.