SARS Nucleocapsid Protein Antibody (14B3D) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07063

Novus Biologicals, part of Bio-Techne
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NBP3-07063
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Key Product Details

Species Reactivity

Validated:

SARS-CoV

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 14B3D

Format

Azide and BSA Free

Concentration

1 mg/ml

View all SARS Nucleocapsid Protein Antibodies »

Product Summary for SARS Nucleocapsid Protein Antibody (14B3D) - Azide and BSA Free

Immunogen

Recombinant SARS Nucleocapsid Protein (no tag)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SARS Nucleocapsid Protein Antibody (14B3D) - Azide and BSA Free

Western Blot: SARS Nucleocapsid Protein Antibody (14B3D)Azide and BSA Free [NBP3-07063]

Western Blot: SARS Nucleocapsid Protein Antibody (14B3D)Azide and BSA Free [NBP3-07063]

Western Blot: SARS Nucleocapsid Protein Antibody (14B3D) [NBP3-07063] - Western Blot Validation of SARS Nucleocapsid Antibody. Lane 1: SARS-CoV-2 Nucleocapsid protein, Lane 2: recombinant SARS Nucleocapsid protein, Lane 3: MERS Nucleocapsid protein probed with SARS Nucleocapsid Antibody [14B3D], NBP3-07063, at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.

Applications for SARS Nucleocapsid Protein Antibody (14B3D) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1000

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.01M PBS (pH7.4)

Format

Azide and BSA Free

Preservative

0.1% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS Nucleocapsid Protein

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139 kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Alternate Names

N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome

Gene Symbol

N

Additional SARS Nucleocapsid Protein Products

Product Documents for SARS Nucleocapsid Protein Antibody (14B3D) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS Nucleocapsid Protein Antibody (14B3D) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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