SARS Nucleocapsid Protein Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56683
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
SARS-CoV, SARS-CoV-2
Cited:
Human, Hamster, Virus - SARS-CoV-2
Applications
Validated:
Dual RNAscope ISH-IHC, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 354-370 (NKHIDAYKTFPPTEPKK-C) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)
Specificity
The was tested on a human cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight of 46 kDa.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS Nucleocapsid Protein Antibody - BSA Free
Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683]
Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56683] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S [848561]); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.
Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683]
Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows lysates of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100). A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) only in the SARS-CoV-2 lysate using 25 ug/mL of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Note: some reactivity observed with FL Std 230. SARS-CoV-2 lysate courtesy of University of Maryland.Applications for SARS Nucleocapsid Protein Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 16014910)
Simple Western
1:50
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SARS Nucleocapsid Protein
Alternate Names
N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome, COVID-19 nucleocapsid protein, SARSCOV2 N protein, SARS-COV-2 nucleocapsid protein, SARS-COV-2 Nucleoprotein
Gene Symbol
N
UniProt
Additional SARS Nucleocapsid Protein Products
Product Documents for SARS Nucleocapsid Protein Antibody - BSA Free
Product Specific Notices for SARS Nucleocapsid Protein Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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