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SART3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74648

Novus Biologicals, part of Bio-Techne
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NB100-74648

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human squamous cell carcinoma antigen recognized by T-cells 3 (Tat-interacting protein of 110 kDa) (NP_055521.1).

Localization

Cytoplasmic and Nuclear. Localized in speckles.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SART3 Antibody

Western Blot: SART3 Antibody [NB100-74648]

Western Blot: SART3 Antibody [NB100-74648]

Western Blot: SART3 Antibody [NB100-74648] - Detection of Human SART3/TIP110 on HeLa whole cell lysate using NB100-74648. SART3 was also immunoprecipitated by rabbit anti-SART3 antibody NB100-74649.
Immunocytochemistry/ Immunofluorescence: SART3 Antibody [NB100-74648]

Immunocytochemistry/ Immunofluorescence: SART3 Antibody [NB100-74648]

Immunocytochemistry/Immunofluorescence: SART3 Antibody [NB100-74648] - FFPE section of human pancreatic islet cell tumor. Affinity purified rabbit anti-SART3 used at a dilution of 1:100. Detection: Red fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100.
Immunohistochemistry: SART3 Antibody [NB100-74648]

Immunohistochemistry: SART3 Antibody [NB100-74648]

Immunohistochemistry: SART3 Antibody [NB100-74648] - Sample: FFPE sections of human ovarian carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- SART3/TIP110 used at a dilution of 1:200 (1ug/ml) and 1:400 (0.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG

Applications for SART3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250- 1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SART3

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.

Alternate Names

DSAP1, hSART-3, KIAA0156squamous cell carcinoma antigen recognised by T cells 3, P100, p110, p110(nrb), RP11-13G14, SART-3, squamous cell carcinoma antigen recognized by T cells 3, squamous cell carcinoma antigen recognized by T-cells 3, Tat-interacting protein of 110 kDa, Tip110, TIP110MGC138188

Entrez Gene IDs

9733 (Human)

Gene Symbol

SART3

UniProt

Additional SART3 Products

Product Documents for SART3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SART3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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