Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunoblotting, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SASH1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human SAM and SH3 domain containing 1 (NP_056093.3).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23235829).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SASH1 Antibody

Western Blot: SASH1 Antibody [NBP1-26650]

Western Blot: SASH1 Antibody [NBP1-26650]

Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate (50 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: SASH1 Antibody [NBP1-26650]

Western Blot: SASH1 Antibody [NBP1-26650]

Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate from HeLa cells. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody NBP1-26651.

Applications for SASH1 Antibody

Application
Recommended Usage

Immunohistochemistry

Reported in scientific literature (PMID: 31067462)

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP1-26650 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SASH1

SASH1 (SAM and SH3 domain containing 1) was identified in an in silico analysis of loss of heterozygosity (LOH) to identify genes downregulated in breast cancer. Since this discovery, it has also been found to be a candidate tumor suppressor in colon cancer. Structurally, SASH1 contains two SAM (sterile alpha motif) domains and one SH3 domain indicating a role in signal transduction and as a signaling adapter.

Alternate Names

dJ323M4, dJ323M4.1, KIAA0790RP3-323M4.1, PEPE1, Proline-glutamate repeat-containing protein, SAM and SH3 domain containing 1,2500002E12Rik, SAM and SH3 domain-containing protein 1, SH3D6A

Entrez Gene IDs

23328 (Human)

Gene Symbol

SASH1

UniProt

O94885 (Human)

Additional SASH1 Products

Product Documents for SASH1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SASH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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