Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SASH1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human SAM and SH3 domain containing 1 using the numbering given in entry NP_056093.3 (GeneID 23328).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SASH1 Antibody

Immunohistochemistry: SASH1 Antibody [NBP1-26651]

Immunohistochemistry: SASH1 Antibody [NBP1-26651]

Immunohistochemistry: SASH1 Antibody [NBP1-26651] - Sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and lightchain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.
Immunoprecipitation: SASH1 Antibody [NBP1-26651]

Immunoprecipitation: SASH1 Antibody [NBP1-26651]

Immunoprecipitation: SASH1 Antibody [NBP1-26651] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit used for IP at 3 mcg/mg lysate. SASH1 was also immunoprecipitated by rabbit anti- SASH1 antibody NBP1-26650, which recognizes an upstream epitope.

Applications for SASH1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250-1:1000

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

3-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SASH1

SASH1 (SAM and SH3 domain containing 1) was identified in an in silico analysis of loss of heterozygosity (LOH) to identify genes downregulated in breast cancer. Since this discovery, it has also been found to be a candidate tumor suppressor in colon cancer. Structurally, SASH1 contains two SAM (sterile alpha motif) domains and one SH3 domain indicating a role in signal transduction and as a signaling adapter.

Alternate Names

dJ323M4, dJ323M4.1, KIAA0790RP3-323M4.1, PEPE1, Proline-glutamate repeat-containing protein, SAM and SH3 domain containing 1,2500002E12Rik, SAM and SH3 domain-containing protein 1, SH3D6A

Entrez Gene IDs

23328 (Human)

Gene Symbol

SASH1

UniProt

NP_056093.3 (Human)

Additional SASH1 Products

Product Documents for SASH1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SASH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.