Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI13D6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SATB1 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SATB1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SATB1 Antibody (OTI13D6) - Azide and BSA Free

Western Blot: SATB1 Antibody (OTI13D6)Azide and BSA Free [NBP2-73991]

Western Blot: SATB1 Antibody (OTI13D6)Azide and BSA Free [NBP2-73991]

Western Blot: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.
Immunocytochemistry/ Immunofluorescence: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991]

Immunocytochemistry/ Immunofluorescence: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991]

Immunocytochemistry/Immunofluorescence: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1.
Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991]

Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991]

Immunohistochemistry: SATB1 Antibody (OTI13D6) - Azide and BSA Free [NBP2-73991] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-SATB1 mouse monoclonal antibody.
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Applications for SATB1 Antibody (OTI13D6) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SATB1

Long Name

SATB Homeobox 1

Alternate Names

DNA-binding protein SATB1, SATB homeobox 1, special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's), Special AT-rich sequence-binding protein 1

Gene Symbol

SATB1

Additional SATB1 Products

Product Documents for SATB1 Antibody (OTI13D6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SATB1 Antibody (OTI13D6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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