Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SATB2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of the human SATB2. The immunogen is located within the first 50 amino acids of SATB2. Amino Acid Squence: CLRDSPDRRSGSPDVK

Specificity

At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SATB2 Antibody - BSA Free

Western Blot: SATB2 AntibodyBSA Free [NBP1-76912]

Western Blot: SATB2 AntibodyBSA Free [NBP1-76912]

Western Blot: SATB2 Antibody [NBP1-76912] - Mouse brain tissue lysate with SATB2 antibody at (A) 2 and (B) 4 ug/mL.
Immunocytochemistry/ Immunofluorescence: SATB2 Antibody - BSA Free [NBP1-76912]

Immunocytochemistry/ Immunofluorescence: SATB2 Antibody - BSA Free [NBP1-76912]

Immunocytochemistry/Immunofluorescence: SATB2 Antibody [NBP1-76912] - Immunofluorescence of SATB2 in Human Brain cells with SATB2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: SATB2 Antibody - BSA Free [NBP1-76912]

Immunohistochemistry-Paraffin: SATB2 Antibody - BSA Free [NBP1-76912]

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-76912] - Human brain with SATB2 antibody at 5 ug/ml.
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Applications for SATB2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2 - 4 ug/mL
Application Notes
SATB2 antibody validated for Chromatin Immunoprecipitation from a verified customer review

Reviewed Applications

Read 1 review rated 4 using NBP1-76912 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SATB2

Human special AT-rich sequence-binding protein-2 (SATB2) is a nuclear matrix/scaffold-associated region DNA-binding protein. Like its homolog SATB1, SATB2 selectively binds double-stranded, special AT-rich DNA sequences, but is expressed primarily in a subset of postmitotic, differentiating neurons in the neocortex. Mice deficient in SATB exhibit craniofacial abnormalities and defects in osteoblast differentiation and function. SATB2 also interacts with and enhances the activity of Runx2 and ATF4, two transcription factors that regulate osteoblast differentiation, indicating that SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Recent experiments have shown that SATB2 interacts with histone deacetylase 1 and metastasis-associated protein 2, two proteins that are involved in chromatin remodeling, suggesting that SATB2 may also be involved in mediating epigenetic influences during cortical development. At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.

Alternate Names

DNA-binding protein SATB2, FLJ32076, KIAA1034FLJ21474, MGC119474, MGC119477, SATB family member 2, SATB homeobox 2, Special AT-rich sequence-binding protein 2

Gene Symbol

SATB2

UniProt

Q9UPW6 (Human)

Additional SATB2 Products

Product Documents for SATB2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SATB2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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