SC35 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47290

Novus Biologicals, part of Bio-Techne
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NBP2-47290
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SC35 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LKVDNLTYRTSPDSLRRVFEKYGRVGDVYIPR

Reactivity Notes

Reactivity reported in scientific literature (PMID: 25800673)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SC35 Antibody

Immunocytochemistry/ Immunofluorescence: SC35 Antibody [NBP2-47290]

Immunocytochemistry/ Immunofluorescence: SC35 Antibody [NBP2-47290]

Immunocytochemistry/Immunofluorescence: SC35 Antibody [NBP2-47290] - Staining of human cell line U-251 MG shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290]

Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290]

Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.
Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290]

Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290]

Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
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Applications for SC35 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 1 using NBP2-47290 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SC35

SC35 is involved in pre-mRNA splicing and is found in the bodies in the nucleus referred to as speckles, SC 35 domains or splicing factor compartments (SFCs). The SC-35 clone is frequently used as a marker for these bodies, the role of which is controversial. Speckles correspond largely if not entirely to ultrastructures termed interchromatin granule clusters (IGCs). SC35 is excluded from the nucleolus.

Alternate Names

PR264, SC35, SC-35, serine/arginine-rich splicing factor 2, SFRS2, SFRS2A, splicing component, 35 kDa, splicing factor SC35, splicing factor, arginine/serine-rich 2, SR splicing factor 2, SRp30b

Gene Symbol

SRSF2

Additional SC35 Products

Product Documents for SC35 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SC35 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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