SCF/c-kit Ligand Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77030

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Tagged Protein Expression

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SCF/c-kit Ligand Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 18 amino acids near the center of human SCF/c-kit Ligand. The immunogen is located within amino acids 100-150 of SCF/c-kit Ligand. Amino Acid Sequence: KENSSKDLKKSFKSPEPR

Specificity

Human SCF/c-kit Ligand has 3 isoforms, including isoform 1 (273aa, 30.9kD), isoform 2 (245aa, 27.9kD) and isoform 3 (238aa, 26.7kD). Mouse SCF/c-kit Ligand has 2 isoforms, including isoform 1 (273aa, 30.6kD) and isoform 2 (245aa, 27.6kD). Rat SCF/c-kit Ligand also has 2 isoforms, including isoform 1 (273aa, 30.7kD) and isoform 2 (245aa, 27.7kD). NBP1-77030 can detect human and possibly detect mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SCF/c-kit Ligand Antibody - BSA Free

Western Blot: SCF/c-kit Ligand AntibodyBSA Free [NBP1-77030]

Western Blot: SCF/c-kit Ligand AntibodyBSA Free [NBP1-77030]

Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane.Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: SCF/c-kit Ligand AntibodyBSA Free [NBP1-77030]

Western Blot: SCF/c-kit Ligand AntibodyBSA Free [NBP1-77030]

Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Analysis of SCF in A431 cell lysate with SCF antibody at (A) 1 and (B) 2 ug/mL.
Western Blot: SCF/c-kit Ligand AntibodyBSA Free [NBP1-77030]

Western Blot: SCF/c-kit Ligand AntibodyBSA Free [NBP1-77030]

Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 30 ng of human SCF recombinant protein per lane.Antibodies: SCF (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 20kD.
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Applications for SCF/c-kit Ligand Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCF/c-kit Ligand

Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.

Long Name

Stem Cell Factor

Alternate Names

c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF

Entrez Gene IDs

4254 (Human); 17311 (Mouse); 60427 (Rat)

Gene Symbol

KITLG

UniProt

P21583

Additional SCF/c-kit Ligand Products

Product Documents for SCF/c-kit Ligand Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SCF/c-kit Ligand Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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