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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5G4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human SCFD1(NP_057190) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SCFD1 Antibody (OTI5G4) - Azide and BSA Free

Western Blot: SCFD1 Antibody (OTI5G4)Azide and BSA Free [NBP2-74004]

Western Blot: SCFD1 Antibody (OTI5G4)Azide and BSA Free [NBP2-74004]

Western Blot: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCFD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCFD1.
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]

Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]

Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - Staining of paraffin-embedded Human Kidney tissue using anti-SCFD1 mouse monoclonal antibody.
Western Blot: SCFD1 Antibody (OTI5G4)Azide and BSA Free [NBP2-74004]

Western Blot: SCFD1 Antibody (OTI5G4)Azide and BSA Free [NBP2-74004]

Western Blot: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-SCFD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Applications for SCFD1 Antibody (OTI5G4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SCFD1

SCFD1 (sec1 family domain containing 1) belongs to the STXBP-unc/SEC1 family of proteins. SEC1 proteins are involved in synaptic transmission and general secretion. SCFD1 is proposed to play a role in vesicular transport between the endoplasmic reticulum and the Golgi.

Alternate Names

C14orf163, KIAA0917chromosome 14 open reading frame 163, sec1 family domain containing 1, sec1 family domain-containing protein 1, SLY1, SLY1 homolog, sly1p, STXBP1L2RA410, Syntaxin-binding protein 1-like 2, vesicle transport-related protein

Gene Symbol

SCFD1

Additional SCFD1 Products

Product Documents for SCFD1 Antibody (OTI5G4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SCFD1 Antibody (OTI5G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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