Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI5E6

Concentration

1 mg/ml

View all SCMH1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-309 of human SCMH1(NP_036368) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

65.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SCMH1 Antibody (OTI5E6)

Western Blot: SCMH1 Antibody (OTI5E6) [NBP2-45724]

Western Blot: SCMH1 Antibody (OTI5E6) [NBP2-45724]

Western Blot: SCMH1 Antibody (5E6) [NBP2-45724] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCMH1.
Immunohistochemistry: SCMH1 Antibody (OTI5E6) [NBP2-45724]

Immunohistochemistry: SCMH1 Antibody (OTI5E6) [NBP2-45724]

Immunohistochemistry: SCMH1 Antibody (5E6) [NBP2-45724] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SCMH1 Antibody (OTI5E6) [NBP2-45724]

Immunohistochemistry: SCMH1 Antibody (OTI5E6) [NBP2-45724]

Immunohistochemistry: SCMH1 Antibody (5E6) [NBP2-45724] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
View 3 more images

Applications for SCMH1 Antibody (OTI5E6)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SCMH1

Polycomb group genes are essential for maintenance of appropriate expression patterns of developmental master regulators, such as Hox genes, and thus are essential for proper development. SCMH1 (Sex comb on midleg homolog 1) is a constituent of the Polycomb group (PcG) multiprotein PRC1 complex. The PcG PRC1 complex acts via chromatin remodeling and modification of histones.

Alternate Names

polycomb protein SCMH1, Scml3, Sex comb on midleg homolog 1, sex comb on midleg homolog 1 (Drosophila)

Gene Symbol

SCMH1

Additional SCMH1 Products

Product Documents for SCMH1 Antibody (OTI5E6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SCMH1 Antibody (OTI5E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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