Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse, Rat, Primate

Applications

Validated:

Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all SCP1 Antibodies »

Product Specifications

Immunogen

A C-terminal synthetic peptide made to the human SCP1 protein sequence. [UniProt# Q15431]

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCP1 Antibody

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-228]

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-228]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-228] - SCP1 labeled in mouse pachytene preparation (red), using NB 300-228. CDK2 staining, near teleomeres, is also present (green).
Simple Western: SCP1 Antibody [NB300-228]

Simple Western: SCP1 Antibody [NB300-228]

Simple Western: SCP1 Antibody [NB300-228] - Lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Applications for SCP1 Antibody

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 27431324)

Immunocytochemistry/ Immunofluorescence

1:300-1:750

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 26858254)

Simple Western

1:200

Western Blot

reported in scientific literature (PMID 23843895)
Application Notes
This SCP1 antibody does not appear to work in Immunohistochemistry on paraffin-embedded sections. *The investigator should determine the optimal working dilution for a specific application.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 1 using NB300-228 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SCP1

SCP1 (synaptonemal complex protein 1), also called cancer/testis antigen 8 (CT8), belongs to a growing family of cancer/testis antigens (CTA) which includes several proteins (MAGE, GAGE/PAGE/XAGE, NY-ESO-1/LAGE-1, SSX, SPANX, TRAG-3, BAGE, OY-TES-1, CT17, NY-BR-3 and SCP-1) that are not expressed in normal tissues except for testis and, in some cases, placenta. SCP1 is found in a complex with SYCE1 and SYCE2, and is a major component of the transverse filaments of synaptonemal complexes. SCP-1 is established to be expressed selectively during the meiotic prophase of spermatocytes formation and is involved in the pairing of homologous chromosomes during meiosis I. SCP1 is localized in tripartite segments of synaptonemal complexes, between lateral elements in the nucleus, and is found only where the chromosome cores are synapsed. Its N-terminus is found towards the center, whereas, the C-terminus extends well into the lateral domain of the synaptonemal complex. SCP-1 differs from other members of CTA family by its localization on chromosome 1 and it is frequently observed to have variable expression in neoplastic tissues/cell lines including malignant gliomas, breast, renal cell, ovarian, pancreatic and hepatocellular carcinomas. Because of exclusive expression of SCP1 in testis/placenta, it is emerging as an important target for cancer research and therapy.

Alternate Names

Cancer/testis antigen 8, CT8MGC104417, HOM-TES-14, SCP1SCP-1, synaptonemal complex protein 1

Gene Symbol

SYCP1

Additional SCP1 Products

Product Documents for SCP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SCP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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