Scribble Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15110
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Scribble. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
175 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Scribble Antibody
![Immunohistochemistry-Paraffin: Scribble Antibody [NBP2-15110]](https://resources.bio-techne.com/images/products/Scribble-Antibody-Immunohistochemistry-Paraffin-NBP2-15110-img0005.jpg "Immunohistochemistry-Paraffin: Scribble Antibody [NBP2-15110]")
Immunohistochemistry-Paraffin: Scribble Antibody [NBP2-15110]
Immunohistochemistry-Paraffin: Scribble Antibody [NBP2-15110] - Human colon carcinoma. SCRIB antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: Scribble Antibody [NBP2-15110]](https://resources.bio-techne.com/images/products/Scribble-Antibody-Western-Blot-NBP2-15110-img0003.jpg "Western Blot: Scribble Antibody [NBP2-15110]")
Western Blot: Scribble Antibody [NBP2-15110]
Western Blot: Scribble Antibody [NBP2-15110] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Scribble Antibody [NBP2-15110] -
Western Blot: Scribble Antibody [NBP2-15110] -Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SCRIB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for Scribble Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Scribble
Alternate Names
CRIB1, protein scribble homolog, SCRB1, SCRIB1, scribbled homolog (Drosophila), Vartul
Gene Symbol
SCRIB
Additional Scribble Products
Product Documents for Scribble Antibody
Product Specific Notices for Scribble Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.