SCUBE2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77186

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SCUBE2 Antibodies »

Product Summary for SCUBE2 Antibody - BSA Free

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the center of human SCUBE2. The immunogen is located within amino acids 480 - 530 of SCUBE2. Amino Acid Squence: CSPLRNKQQKSNDSAFGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCUBE2 Antibody - BSA Free

Western Blot: SCUBE2 AntibodyBSA Free [NBP1-77186]

Western Blot: SCUBE2 AntibodyBSA Free [NBP1-77186]

Western Blot: SCUBE2 Antibody [NBP1-77186] - Daudi cell lysate with SCUBE2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: SCUBE2 Antibody - BSA Free [NBP1-77186]

Immunocytochemistry/ Immunofluorescence: SCUBE2 Antibody - BSA Free [NBP1-77186]

Immunocytochemistry/Immunofluorescence: SCUBE2 Antibody [NBP1-77186] - Immunofluorescence of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: SCUBE2 Antibody - BSA Free [NBP1-77186]

Immunocytochemistry/ Immunofluorescence: SCUBE2 Antibody - BSA Free [NBP1-77186]

Immunocytochemistry/Immunofluorescence: SCUBE2 Antibody [NBP1-77186] - Daudi cells with SCUBE2 antibody at 20 ug/mL.

Applications for SCUBE2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunofluorescence

20 ug/ml

Western Blot

1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCUBE2

SCUBE2 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and are expressed in embryonic neuroectoderm and vascular epithelium. SCUBE2 is a close homolog to SCUBE1 and can form a heterodimeric complex with SCUBE1. SCUBE2 is thought to participate in the Hedgehog signaling pathway by specifically interacting with Sonic Hedgehog (SHH) and the receptor PTCH1, enhancing the SHH signaling activity in the raft microdomains of the plasma membrane, and may thus play important roles in organ development and tumor progression.

Alternate Names

CEGB1, CEGF1, CEGP1, FLJ16792, FLJ35234, MGC133057, Protein CEGP1, signal peptide, CUB and EGF-like domain-containing protein 2, signal peptide, CUB domain, EGF-like 2

Gene Symbol

SCUBE2

UniProt

NP_066025 (Human)

Additional SCUBE2 Products

Product Documents for SCUBE2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SCUBE2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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