SDHA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32594

Novus Biologicals, part of Bio-Techne
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NBP2-32594
NBP2-32594-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SDHA Antibodies »

Product Summary for SDHA Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ERASAKVSDSISAQYPVVDHEFDAVV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SDHA Antibody

Western Blot: SDHA Antibody [NBP2-32594]

Western Blot: SDHA Antibody [NBP2-32594]

Western Blot: SDHA Antibody [NBP2-32594] - Analysis using Anti-SDHA antibody NBP2-32594 (A) shows similar pattern to independent antibody NBP2-56988 (B).
Immunocytochemistry/ Immunofluorescence: SDHA Antibody [NBP2-32594]

Immunocytochemistry/ Immunofluorescence: SDHA Antibody [NBP2-32594]

Immunocytochemistry/Immunofluorescence: SDHA Antibody [NBP2-32594] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SDHA Antibody [NBP2-32594]

Immunohistochemistry-Paraffin: SDHA Antibody [NBP2-32594]

Immunohistochemistry-Paraffin: SDHA Antibody [NBP2-32594] - Staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.

Applications for SDHA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SDHA

The SDHA gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations

Long Name

SDHA

Alternate Names

CMD1GG, MC2DN1, NDAXOA, PGL5, PPGL5, SDH1, SDH2, SDHF

Gene Symbol

SDHA

Additional SDHA Products

Product Documents for SDHA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SDHA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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