SDHA Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93922

Novus Biologicals, part of Bio-Techne
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NBP2-93922-0.02ml
NBP2-93922-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SDHA Antibodies »

Product Summary for SDHA Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 385-664 of human SDHA (NP_004159.2). ETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKISKLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPAIRSY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SDHA Antibody - Azide and BSA Free

Western Blot: SDHA AntibodyAzide and BSA Free [NBP2-93922]

Western Blot: SDHA AntibodyAzide and BSA Free [NBP2-93922]

Western Blot: SDHA Antibody [NBP2-93922] - Western blot analysis of extracts of various cell lines, using SDHA antibody (NBP2-93922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: SDHA Antibody - Azide and BSA Free [NBP2-93922]

Immunocytochemistry/ Immunofluorescence: SDHA Antibody - Azide and BSA Free [NBP2-93922]

Immunocytochemistry/Immunofluorescence: SDHA Antibody [NBP2-93922] - Immunofluorescence analysis of U2OS cells using SDHA Rabbit pAb (NBP2-93922) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SDHA Antibody - Azide and BSA Free [NBP2-93922]

Immunohistochemistry-Paraffin: SDHA Antibody - Azide and BSA Free [NBP2-93922]

Immunohistochemistry-Paraffin: SDHA Antibody [NBP2-93922] - Immunohistochemistry of paraffin-embedded human colon carcinoma using SDHA Rabbit pAb (NBP2-93922) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for SDHA Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SDHA

The SDHA gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations

Long Name

SDHA

Alternate Names

CMD1GG, MC2DN1, NDAXOA, PGL5, PPGL5, SDH1, SDH2, SDHF

Gene Symbol

SDHA

Additional SDHA Products

Product Documents for SDHA Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SDHA Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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