SDHB Antibody (7D12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26332
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7D12
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SDHB Antibody (7D12)
Immunogen
A synthesized peptide derived from Human SDHB [UniProt P21912]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SDHB Antibody (7D12)
Immunohistochemistry: SDHB Antibody (7D12) [NBP3-26332] -
Immunohistochemistry: SDHB Antibody (7D12) [NBP3-26332] - Image of SDHB Antibody (7D12) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: SDHB Antibody (7D12) [NBP3-26332] -
Immunohistochemistry: SDHB Antibody (7D12) [NBP3-26332] - Image of SDHB Antibody (7D12) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: SDHB Antibody (7D12) [NBP3-26332] -
Western Blot: SDHB Antibody (7D12) [NBP3-26332] - Positive Western Blot detected in: 293 whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate, PC-3 whole cell lysate, Rat heart tissue, Mouse heart tissue, Mouse liver tissue.All lanes: SDHB Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Applications for SDHB Antibody (7D12)
Application
Recommended Usage
Flow Cytometry
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: SDHB
Alternate Names
EC 1.3.5.1, FLJ92337, Ip, Iron-sulfur subunit of complex II, PGL4, SDH, SDH1, SDH2, SDHIP, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Gene Symbol
SDHB
Additional SDHB Products
Product Documents for SDHB Antibody (7D12)
Product Specific Notices for SDHB Antibody (7D12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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