Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SDHB/6697R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all SDHB Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues in human SDHB was used as an immunogen. (Uniprot: P21912 )

Localization

Cytoplasm, Mitochondria

Marker

Pheochromocytoma Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07818).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for SDHB Antibody (SDHB/6697R) - Azide and BSA Free

Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/6697R) - Azide and BSA Free [NBP3-08827]

Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/6697R) - Azide and BSA Free [NBP3-08827]

Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/6697R) - Azide and BSA Free [NBP3-08827] - Formalin-fixed, paraffin-embedded human paraganglioma stained with SDHB Recombinant Rabbit Monoclonal Antibody (SDHB/6697R).
Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/6697R) - Azide and BSA Free [NBP3-08827]

Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/6697R) - Azide and BSA Free [NBP3-08827]

Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/6697R) - Azide and BSA Free [NBP3-08827] - Formalin-fixed, paraffin-embedded human heart stained with SDHB Recombinant Rabbit Monoclonal Antibody (SDHB/6697R).

Applications for SDHB Antibody (SDHB/6697R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: SDHB

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Alternate Names

EC 1.3.5.1, FLJ92337, Ip, Iron-sulfur subunit of complex II, PGL4, SDH, SDH1, SDH2, SDHIP, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Gene Symbol

SDHB

Additional SDHB Products

Product Documents for SDHB Antibody (SDHB/6697R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SDHB Antibody (SDHB/6697R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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