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SDHB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-793

Novus Biologicals, part of Bio-Techne
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NB600-793

Key Product Details

Species Reactivity

Human

Applications

Control, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-ATYKEKKASV corresponding to C-Terminus according to NP_002991.2.

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SDHB Antibody

Immunocytochemistry/ Immunofluorescence: SDHB Antibody [NB600-793]

Immunocytochemistry/ Immunofluorescence: SDHB Antibody [NB600-793]

Immunocytochemistry/Immunofluorescence: SDHB Antibody [NB600-793] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry: SDHB Antibody [NB600-793]

Immunohistochemistry: SDHB Antibody [NB600-793]

Immunohistochemistry: SDHB Antibody [NB600-793] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: SDHB Antibody [NB600-793]

Flow Cytometry: SDHB Antibody [NB600-793]

Flow Cytometry: SDHB Antibody [NB600-793] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for SDHB Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

6.0 - 8.0 ug/ml

Immunohistochemistry-Paraffin

6.0 - 8.0 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
Western blot: Approx 28-30kDa band observed in lysates of cell lines HEK293, Jurkat and HeLa (calculated MW of 31.6kDa according to NP_002991.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SDHB

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme.

Alternate Names

EC 1.3.5.1, FLJ92337, Ip, Iron-sulfur subunit of complex II, PGL4, SDH, SDH1, SDH2, SDHIP, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Entrez Gene IDs

6390 (Human)

Gene Symbol

SDHB

Additional SDHB Products

Product Documents for SDHB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SDHB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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