SEC14L2 Antibody (OTI4H2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74051

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4H2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SEC14L2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SEC14L2(NP_036561) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SEC14L2 Antibody (OTI4H2) - Azide and BSA Free

Western Blot: SEC14L2 Antibody (OTI4H2)Azide and BSA Free [NBP2-74051]

Western Blot: SEC14L2 Antibody (OTI4H2)Azide and BSA Free [NBP2-74051]

Western Blot: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.
Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051]

Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051]

Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2.
Immunohistochemistry: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051]

Immunohistochemistry: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051]

Immunohistochemistry: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SEC14L2 mouse monoclonal antibody.
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Applications for SEC14L2 Antibody (OTI4H2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SEC14L2

SEC14L2 encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway.

Alternate Names

C22orf6, hTAP, KIAA1186SEC14 (S. cerevisiae)-like 2, KIAA1658SPFAlpha-tocopherol-associated protein, SEC14-like 2 (S. cerevisiae), SEC14-like protein 2, Squalene transfer protein, Supernatant protein factor, TAP1, TAPMGC65053, tocopherol-associated protein

Gene Symbol

SEC14L2

Additional SEC14L2 Products

Product Documents for SEC14L2 Antibody (OTI4H2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SEC14L2 Antibody (OTI4H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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