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SEC16A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83016

Novus Biologicals, part of Bio-Techne
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NBP1-83016-25ul
NBP1-83016

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLPIPSNLFVPTPDAEEPQLPDGTGREGPAAARGLANPEPAPEPKAPGDLPAAGGPPSGAMPFYNPAQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SEC16A Antibody

Immunocytochemistry/ Immunofluorescence: SEC16A Antibody [NBP1-83016]

Immunocytochemistry/ Immunofluorescence: SEC16A Antibody [NBP1-83016]

Immunocytochemistry/Immunofluorescence: SEC16A Antibody [NBP1-83016] - Staining of human cell line A-431 shows localization to endoplasmic reticulum & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SEC16A Antibody [NBP1-83016]

Immunohistochemistry-Paraffin: SEC16A Antibody [NBP1-83016]

Immunohistochemistry-Paraffin: SEC16A Antibody [NBP1-83016] - Staining of human skin shows strong cytoplasmic granular positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: SEC16A Antibody [NBP1-83016]

Immunohistochemistry-Paraffin: SEC16A Antibody [NBP1-83016]

Immunohistochemistry-Paraffin: SEC16A Antibody [NBP1-83016] - Staining of human pancreas shows strong cytoplasmic granular positivity in exocrine glandular cells.

Applications for SEC16A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SEC16A

SEC16A is required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. It defines endoplasmic reticulum exit sites (ERES). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. It is required for normal transitional endoplasmic reticulum (tER) organization.

Alternate Names

FLJ26737, KIAA0310RP11-413M3.10, p250protein SEC16 homolog A, SEC16, SEC16 homolog A, SEC16 homolog A (S. cerevisiae), SEC16Lprotein transport protein Sec16A

Gene Symbol

SEC16A

Additional SEC16A Products

Product Documents for SEC16A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SEC16A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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