SEC62 Antibody (PSH0-91)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32940
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH0-91
Concentration
1 mg/ml
Product Summary for SEC62 Antibody (PSH0-91)
Immunogen
Synthetic peptide within human SEC62 aa 71-120 / 399. (Uniprot: Q99442)
Localization
Endoplasmic reticulum membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SEC62 Antibody (PSH0-91)
Western Blot: SEC62 Antibody (PSH0-91) [NBP3-32940]
Western Blot: SEC62 Antibody (PSH0-91) [NBP3-32940] - Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (NBP3-32940) at 1/1,000 dilution.Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: K-562 cell lysate (20 ug/Lane)
Lane 3: SH-SY5Y cell lysate (20 ug/Lane)
Lane 4: HepG2 cell lysate (20 ug/Lane)
Lane 5: 293T cell lysate (20 ug/Lane)
Lane 6: Jurkat cell lysate (20 ug/Lane)
Lane 7: MCF7 cell lysate (20 ug/Lane)
Lane 8: Daudi cell lysate (20 ug/Lane)
Lane 9: PC-12 cell lysate (20 ug/Lane)
Lane 10: Mouse liver tissue lysate (40 ug/Lane)
Lane 11: Mouse brain tissue lysate (40 ug/Lane)
Lane 12: Rat spleen tissue lysate (40 ug/Lane)
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32940) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for SEC62 Antibody (PSH0-91)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SEC62
Alternate Names
Dtrp1, hTP-1, S.cerevisiae, homolog of, SEC62 homolog (S. cerevisiae), TLOC1, TP-1, translocation protein 1, translocation protein SEC62
Gene Symbol
SEC62
Additional SEC62 Products
Product Documents for SEC62 Antibody (PSH0-91)
Product Specific Notices for SEC62 Antibody (PSH0-91)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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