Sec8 Antibody (14G1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97492

Novus Biologicals, part of Bio-Techne
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NBP1-97492-0.025mg
NBP1-97492-0.05mg
NBP1-97492-0.2mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Fish, Guinea Pig, Hamster, Primate, Rabbit, Sheep

Cited:

Mouse, Rat, Canine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 14G1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sec8 Antibodies »

Product Specifications

Immunogen

Recombinant rat rSec8.

Reactivity Notes

Human, Mouse, Rat, Bovine, Canine, Chicken, Fish, Guinea Pig, Hamster, Primate, Pig, Rabbit and Sheep. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Sec8 Antibody (14G1)

Western Blot: Sec8 Antibody (14G1) [NBP1-97492]

Western Blot: Sec8 Antibody (14G1) [NBP1-97492]

Western Blot: Sec8 Antibody (14G1) [NBP1-97492] - Lane 1: MWM, Lane 2: Brain (rat), (tissue extract), Lane 3: Brain (mouse), (tissue extract), Lane 4: MDBK Cell Lysate.
Immunohistochemistry-Paraffin: Sec8 Antibody (14G1) [NBP1-97492]

Immunohistochemistry-Paraffin: Sec8 Antibody (14G1) [NBP1-97492]

Immunohistochemistry-Paraffin: Sec8 Antibody (14G1) [NBP1-97492] - Analysis of human brain, cerebellum tissue stained with rSec8, mAb (14G1) at 5ug/ml.

Applications for Sec8 Antibody (14G1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

12.5 ug/ml

Western Blot

1:1000
Application Notes
This antibody is useful in Immunocytochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation and detects a band of ~110kDa by Western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sec8

rSec6 and rSec8 are rat homologs of the yeast Sec6 and Sec8 proteins that are essential for protein transport between the Golgi apparatus and the plasma membrane. In yeast, Sec8 is part of a 19.5S complex that includes Sec6 and Sec15, that also associate with the ras-like GTP binding protein Sec4. An analogous 17S complex in rat brain was found to contain rSec6 and rSec8, along with eight proteins with a combined molecular weight of 743 kDa. The membrane-associated rSec6/8 complex co-immunoprecipitates with syntaxin, providing evidence that this complex is involved in synaptic vesicle docking and fusion at the presynaptic terminal.

Alternate Names

exocyst complex component 4, KIAA1699SEC8L1, MGC27170, REC8, SEC8Exocyst complex component Sec8, SEC8-like 1, SEC8-like 1 (S. cerevisiae), Sec8p

Gene Symbol

EXOC4

Additional Sec8 Products

Product Documents for Sec8 Antibody (14G1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Sec8 Antibody (14G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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