Secretagogin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20286
![Western Blot: Secretagogin Antibody [NBP2-20286]](https://resources.bio-techne.com/images/products/Secretagogin-Antibody-Western-Blot-NBP2-20286-img0005.jpg "Western Blot: Secretagogin Antibody [NBP2-20286]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Secretagogin. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (84%), Xenopus tropicalis (84%).
Localization
Cytoplasm; Secreted (By similarity); Cytoplasmic vesicle; secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Secretagogin Antibody
![Western Blot: Secretagogin Antibody [NBP2-20286]](https://resources.bio-techne.com/images/products/Secretagogin-Antibody-Western-Blot-NBP2-20286-img0009.jpg "Western Blot: Secretagogin Antibody [NBP2-20286]")
Western Blot: Secretagogin Antibody [NBP2-20286]
Western Blot: Secretagogin Antibody [NBP2-20286] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Secretagogin antibody diluted at 1:500.![Immunocytochemistry/ Immunofluorescence: Secretagogin Antibody [NBP2-20286]](https://resources.bio-techne.com/images/products/Secretagogin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20286-img0008.jpg "Immunocytochemistry/ Immunofluorescence: Secretagogin Antibody [NBP2-20286]")
Immunocytochemistry/ Immunofluorescence: Secretagogin Antibody [NBP2-20286]
Immunocytochemistry/Immunofluorescence: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Secretagogin stained by Secretagogin antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]](https://resources.bio-techne.com/images/products/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0010.jpg "Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]")
Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]
Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Secretagogin protein stained by Secretagogin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.![Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP2-20286]](https://resources.bio-techne.com/images/products/Secretagogin-Antibody-Immunohistochemistry-Paraffin-NBP2-20286-img0002.jpg "Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP2-20286]")
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]](https://resources.bio-techne.com/images/products/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0006.jpg "Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]")
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]](https://resources.bio-techne.com/images/products/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0007.jpg "Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286]")
Applications for Secretagogin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Secretagogin
Additional Secretagogin Products
Product Documents for Secretagogin Antibody
Product Specific Notices for Secretagogin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.