Seladin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38980

Novus Biologicals, part of Bio-Techne
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NBP2-38980
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Seladin 1 Antibodies »

Product Summary for Seladin 1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ILEVDTKKQIVRVEPLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRCTPSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Seladin 1 Antibody

Western Blot: Seladin 1 Antibody [NBP2-38980]

Western Blot: Seladin 1 Antibody [NBP2-38980]

Western Blot: Seladin 1 Antibody [NBP2-38980] - Analysis in control (vector only transfected HEK293T lysate) and DHCR24 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry: Seladin 1 Antibody [NBP2-38980]

Immunohistochemistry: Seladin 1 Antibody [NBP2-38980]

Immunohistochemistry: Seladin 1 Antibody [NBP2-38980] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.

Applications for Seladin 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Seladin 1

Seladin 1 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.

Alternate Names

24-dehydrocholesterol reductaseNbla03646, DCE, desmosterol-to-cholesterol enzyme, Diminuto/dwarf1 homolog, EC 1.3.1.72, KIAA0018SELADIN1, seladin 1,3-beta-hydroxysterol delta-24-reductase, seladin-1, selective AD indicator 1,3 beta-hydroxysterol delta 24-reductase

Gene Symbol

DHCR24

UniProt

Q15392 (Human)

Additional Seladin 1 Products

Product Documents for Seladin 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Seladin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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