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Semaphorin 3A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23380

Novus Biologicals, part of Bio-Techne
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NBP3-23380

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminal region of human Semaphorin 3A. The sequence used is identical to similar regions in rat and mouse Semaphorin 3A, and has low homology to other semaphorin family members. [UniProt# Q14563]

Specificity

The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Semaphorin 3A on SDS-PAGE immunoblots of human recombinant Sema3A/Fc chimera. This antibody has not been shown to be reactive in neonatal rat brain, but has weak reactivity in adult mouse brain.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Semaphorin 3A Antibody

Semaphorin 3A Antibody

Western Blot: Semaphorin 3A Antibody [NBP3-23380] -

Western Blot: Semaphorin 3A Antibody [NBP3-23380] - Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Semaphorin 3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Semaphorin 3A (NBP3-23380) (lanes 1 & 2), anti-Semaphorin 3A (NBP3-23223) (lanes 3 & 4) and anti-Semaphorin 3A (NBP3-23379) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Semaphorin 3A.
Semaphorin 3A Antibody

Immunocytochemistry/Immunofluorescence: Semaphorin 3A Antibody [NBP3-23380] -

Immunocytochemistry/Immunofluorescence: Semaphorin 3A Antibody [NBP3-23380] - Immunocytochemical labeling of Sema-3A in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal Sema-3A (N-terminal) antibody (NBP3-23380), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.

Applications for Semaphorin 3A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:400

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Semaphorin 3A

SEMA3A - sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A

Alternate Names

Sema3A

Gene Symbol

SEMA3A

Additional Semaphorin 3A Products

Product Documents for Semaphorin 3A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Semaphorin 3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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