Semaphorin 3F Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23386

Novus Biologicals, part of Bio-Techne
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NBP3-23386
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Semaphorin 3F Antibodies »

Product Specifications

Immunogen

Semaphorin 3F synthetic peptide (coupled to carrier protein) corresponds to amino acids from the C-terminal region of human Sema-3F. This sequence is highly conserved in rat and mouse Sema-3F, and has low homology to other semaphorin family members. [UniProt# Q13275]

Specificity

The antibody detects a 95 kDa protein corresponding to the apparent molecular mass of Semaphorin 3F on SDS-PAGE immunoblots of adult mouse brain and liver.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Semaphorin 3F Antibody

Semaphorin 3F Antibody

Western Blot: Semaphorin 3F Antibody [NBP3-23386] -

Western Blot: Semaphorin 3F Antibody [NBP3-23386] - Western blot analysis of Semaphorin 3F expression in adult mouse brain (lane 1), kidney (lane 2), and liver (lane 3). The blot was probed with anti-Semaphorin 3F (NBP3-23386) at 1:1000.

Applications for Semaphorin 3F Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Semaphorin 3F

The Semaphorins make up the largest family of axon guidance cues yet described. Semaphorins are divided into 8 classes (classes 3-7 found in vertebrates). Class 3 Semaphorins are secreted, classes 4 through 6 are transmembrane proteins, and class 7 are membrane associated via glycosylphosphatidylinositol (GPI) linkage. They are characterized structurally by a conserved ~400 amino acid sema domain. They are classically described as collapsing factors and mediators of axon repulsion, although they may also act as context-dependent chemoattractants. Semaphorins have been shown to have roles in cardiovascular development and in the regulation of immune cell antigen presentation. Receptors or receptor complexes that mediate semaphorin signaling include proteins of the Neuropilin and Plexin families.

Alternate Names

Sema3F, SEMA4, SEMAK

Gene Symbol

SEMA3F

Additional Semaphorin 3F Products

Product Documents for Semaphorin 3F Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Semaphorin 3F Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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