Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28669
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG4 Clone # pepinemab
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
SEMA4D / CD100
Clonality
Monoclonal
Host
Human
Isotype
IgG4
Endotoxin Level
< 0.001EU/ug,determined by LAL method.
Description
Expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
Also known as 'pepinemab'.
IgG4SP is human IgG4 with S228P mutation.
Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.
Also known as 'pepinemab'.
IgG4SP is human IgG4 with S228P mutation.
Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP
In vivo assay: Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP[NBP3-28669] -
Pepinemab inhibited the tumor growth of CT26 on balb/c mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 76.9% at 50 mpk at D24.
ELISA: Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP[NBP3-28669] -
Immobilized human SEMA4D/CD100, His Tag at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP, EC50=0.0081 ug/mL.
Flow Cytometry: Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP[NBP3-28669] -
Human C100 HEK293 cells were stained with Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.3227 nM.Applications for Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.
Formulation
Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Semaphorin 4D/CD100
Additional Semaphorin 4D/CD100 Products
Product Documents for Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP
Product Specific Notices for Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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