Senataxin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-94712

Novus Biologicals, part of Bio-Techne
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NBP1-94712
NBP1-94712SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Senataxin Antibodies »

Product Specifications

Immunogen

A partial recombinant protein made to an internal portion of the human Senataxin protein (between residues 600-750) [UniProt Q7Z333]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28245518).

Localization

Nucleus, nucleoplasm, nucleolus. Cytoplasm.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Senataxin Antibody - BSA Free

Western Blot: Senataxin AntibodyBSA Free [NBP1-94712]

Western Blot: Senataxin AntibodyBSA Free [NBP1-94712]

Western Blot: Senataxin Antibody [NBP1-94712] - Input: HeLa whole cell lysate. Beads without antibody IP control. IP: IP from HeLa lysate.
Immunocytochemistry/ Immunofluorescence: Senataxin Antibody - BSA Free [NBP1-94712]

Immunocytochemistry/ Immunofluorescence: Senataxin Antibody - BSA Free [NBP1-94712]

Immunocytochemistry/Immunofluorescence: Senataxin Antibody [NBP1-94712] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Senataxin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Senataxin Antibody - BSA Free [NBP1-94712]

Immunohistochemistry-Paraffin: Senataxin Antibody - BSA Free [NBP1-94712]

Immunohistochemistry-Paraffin: Senataxin Antibody [NBP1-94712] - Analysis of a FFPE tissue section of human heart (transverse section) using 1:200 dilution of lot A2 of Senataxin antibody. The antibody generated a very strong staining in the cytoplasm and the nuclei of the muscle cells. No signal was found in the perimysium and endomycium area (connective tissue) of the section.
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Applications for Senataxin Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Immunoprecipitation

1:500

Western Blot

1:500
Application Notes
In ICC/IF, nuclear staining was observed in HeLa cells. In IP and Western blot a band is seen at ~300 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-94712 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Senataxin

Senataxin (SETX, ALS4, AOA2, SCAR1) belongs to the DNA/NAM7 helicase family. The protein encoded for by Senataxin has a DNA/RNA helicase domain at its C-terminal end. Senataxin may play a role in RNA maturation. It may also be involved in DNA double-strand breaks damage response caused by oxidative stress. Cellular localization of Senataxin is limited to the nucleus and cytoplasm. Senataxin is abundantly expressed in skeletal muscle. Expression of Senataxin is also seen in heart, fibroblast, placenta, liver, and weakly in brain and lung. Post translational modifications of Senataxin include acetylation and phosphorylation. There are 3 known isoforms (302.9, 299.4 and 306.3 kDa).

Alternate Names

ALS4recessive, non-Friedreich type 1, AOA2FLJ43459, DKFZp781B151, KIAA0625FLJ12840, senataxin, SETX

Entrez Gene IDs

23064 (Human)

Gene Symbol

SETX

UniProt

Q7Z333 (Human)

Additional Senataxin Products

Product Documents for Senataxin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Senataxin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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