Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SENP3 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 275 and 325 of human SUMO1/Sentrin/SMT3 Specific Peptidase 3 using the numbering given in entry NP_056485.2 (GeneID 26168).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SENP3 Antibody

Western Blot: SENP3 Antibody [NBP1-71895]

Western Blot: SENP3 Antibody [NBP1-71895]

Western Blot: SENP3 Antibody [NBP1-71895] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-SENP3 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SENP3 was also immunoprecipitated by rabbit anti-SENP3 antibody NBP1-71894, which recognizes an upstream epitope.
Immunohistochemistry: SENP3 Antibody [NBP1-71895]

Immunohistochemistry: SENP3 Antibody [NBP1-71895]

Immunohistochemistry: SENP3 Antibody [NBP1-71895] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- SENP3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Applications for SENP3 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SENP3

SENP3 is a protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. SENP3 deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability.

Alternate Names

DKFZP586K0919, DKFZp762A152, EC 3.4.22, EC 3.4.22.-, sentrin/SUMO-specific protease 3, Sentrin/SUMO-specific protease SENP3, sentrin-specific protease 3, SMT3IP1, SSP3DKFZp586K0919, SUMO1/sentrin/SMT3 specific peptidase 3, SUMO1/sentrin/SMT3 specific protease 3, SUMO-1-specific protease 3, SUSP3

Gene Symbol

SENP3

UniProt

NP_056485.2 (Human)

Additional SENP3 Products

Product Documents for SENP3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SENP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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