Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SENP7 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 650 and 700 of human SUMO1/Sentrin Specific Peptidase 7 using the numbering given in entry EAW79800.1 (GeneID 57337).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SENP7 Antibody

Western Blot: SENP7 Antibody [NBP1-50027]

Western Blot: SENP7 Antibody [NBP1-50027]

Western Blot: SENP7 Antibody [NBP1-50027] - Detection of Human SENP7 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SENP7 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SENP7 was also immunoprecipitated by rabbit anti-SENP7 antibody BL10697, which recognizes an upstream epitope. For blotting immunoprecipitated SENP7, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for SENP7 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SENP7

SAE1, or SUMO-1 specific protease 2 is a ubiquitin-like (Ubl) protein that has been shown to bind to several protiens including Ran GTPase-activating protein 1 (RanGAP1), IkappaBalpha, and PML. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes.

Alternate Names

EC 3.4.22.-, KIAA1707SUMO1/sentrin specific protease 7, MGC157730, Sentrin/SUMO-specific protease SENP7, sentrin-specific protease 7, SSP2, SUMO1/sentrin specific peptidase 7, SUMO-1-specific protease 2, SUSP2

Entrez Gene IDs

57337 (Human)

Gene Symbol

SENP7

UniProt

Q9BQF6 (Human)

Additional SENP7 Products

Product Documents for SENP7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SENP7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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