Separase Antibody (XJ11-4D7)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-330

Novus Biologicals, part of Bio-Techne
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NB100-330
NB100-330SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # XJ11-4D7

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Separase Antibodies »

Product Specifications

Immunogen

Maltose-Binding Protein fusion of C-terminal fragment of human Separase (amino acids 1866-1996). [UniProt# Q14674]

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:17626243).

Localization

Cytoplasmic and Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Separase Antibody (XJ11-4D7)

Immunocytochemistry/ Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330]

Immunocytochemistry/ Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330]

Immunocytochemistry/Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330] - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using NB 100-330, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.

Applications for Separase Antibody (XJ11-4D7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

reported in scientific literature (PMID 17626243)
Application Notes
In ICC/IF, this antibody recognizes endogenous Separase in paraformaldehyde-fixated preparations of human cells.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Separase

In normal cellular proliferation, the maintenance of constant centriole numbers is strongly associated with cell cycle process and the disengagement of mother-daugther centriole (a requirement for duplication of centriole) is facilitated by separase (ESPL1, a cysteine endopeptidase) mediated proteolytic cleavage of cohesin, a "glue" protein complex which is also essential to sister-chromatide cohesion. Separase is a caspase-like protease which plays a key role in chromosome segregation by cleaving SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle phases, separase is kept inactivated, and its proteolytic activity is tightly regulated by multiple inhibitory mechanisms combining securin binding, CyclinB1/Cdk1 mediated serine residue phosphorylation (pSer1226), PP2A binding and autocatalytic cleavage. Ectopic activation of separase proteolytic activity results in premature sister-chromatide separation/centriole disengagement and separase overexpression has been shown to induce aneuploidy and carcinogenesis.

Alternate Names

ESPL1, PRCE

Entrez Gene IDs

9700 (Human)

Gene Symbol

ESPL1

UniProt

Q14674 (Human)

Additional Separase Products

Product Documents for Separase Antibody (XJ11-4D7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Separase Antibody (XJ11-4D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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