SEPHS2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77776
Key Product Details
Validated by
Biological Validation
Species Reactivity
Mouse
Applications
ELISA, Immunoprecipitation, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This Protein A purified SEPHS2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SEPHS2. (Uniprot: P97364)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with SEPHS2 from human sources based on an 84% homology with the immunizing sequence. Cross-reactivity with SEPHS2 from other sources has not been determined.
Specificity
This antibody reacts with mouse SEPHS2 and shows partial cross-reactivity with SPS1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was purified by Protein A chromatography from monospecific antiserum
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for SEPHS2 Antibody
![Western Blot: SEPHS2 Antibody [NBP1-77776]](https://resources.bio-techne.com/images/products/SEPHS2-Antibody-Western-Blot-NBP1-77776-img0002.jpg "Western Blot: SEPHS2 Antibody [NBP1-77776]")
Western Blot: SEPHS2 Antibody [NBP1-77776]
Western Blot: SEPHS2 Antibody [NBP1-77776] - Shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
SEPHS2 Antibody
Western blot using Protein A purified anti-SPS2 antibody shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.Applications for SEPHS2 Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:2000
Application Notes
This Protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to SPS2 by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: SEPHS2
Alternate Names
EC 2.7.9.3, Selenophosphate synthase 2, selenophosphate synthetase 2, SPS2water dikinase 2
Gene Symbol
SEPHS2
UniProt
Additional SEPHS2 Products
Product Documents for SEPHS2 Antibody
Product Specific Notices for SEPHS2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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