Septin-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85212
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212]](https://resources.bio-techne.com/images/products/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85212-img0018.jpg "Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212]")
![Western Blot: Septin-2 Antibody [NBP1-85212]](https://resources.bio-techne.com/images/products/Septin-2-Antibody-Western-Blot-NBP1-85212-img0014.jpg "Western Blot: Septin-2 Antibody [NBP1-85212]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Cited:
IF/IHC, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: WGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Septin-2 Antibody
![Western Blot: Septin-2 Antibody [NBP1-85212]](https://resources.bio-techne.com/images/products/Septin-2-Antibody-Western-Blot-NBP1-85212-img0019.jpg "Western Blot: Septin-2 Antibody [NBP1-85212]")
Western Blot: Septin-2 Antibody [NBP1-85212]
Western Blot: Septin-2 Antibody [NBP1-85212] - Stable septin-2 knockdown shows a decrease in proliferation. Verification of septin-2 knockdown at protein level visualized by Western blot. A single blot was probed simultaneously for septin-2 and GAPDH as a loading control. The original blot can be seen in Supplementary Figure 4. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.26836), licensed under a CC-BY license.![Immunocytochemistry/ Immunofluorescence: Septin-2 Antibody [NBP1-85212]](https://resources.bio-techne.com/images/products/Septin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85212-img0012.jpg "Immunocytochemistry/ Immunofluorescence: Septin-2 Antibody [NBP1-85212]")
Immunocytochemistry/ Immunofluorescence: Septin-2 Antibody [NBP1-85212]
Immunocytochemistry/Immunofluorescence: Septin-2 Antibody [NBP1-85212] - Imaging of cultured rat corrtical astrocytes. Image submitted by a verified customer review.![Western Blot: Septin-2 Antibody [NBP1-85212]](https://resources.bio-techne.com/images/products/Septin-2-Antibody-Western-Blot-NBP1-85212-img0009.jpg "Western Blot: Septin-2 Antibody [NBP1-85212]")
![Immunocytochemistry/ Immunofluorescence: Septin-2 Antibody [NBP1-85212]](https://resources.bio-techne.com/images/products/Septin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85212-img0011.jpg "Immunocytochemistry/ Immunofluorescence: Septin-2 Antibody [NBP1-85212]")
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212]](https://resources.bio-techne.com/images/products/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85212-img0015.jpg "Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212]")
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212]](https://resources.bio-techne.com/images/products/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85212-img0016.jpg "Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212]")
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212]](https://resources.bio-techne.com/images/products/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85212-img0017.jpg "Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212]")
Applications for Septin-2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200-1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP1-85212 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Septin-2
Alternate Names
DIFF6NEDD-5, hNedd5, KIAA0158Neural precursor cell expressed developmentally down-regulated protein 5, NEDD5Pnutl3, neural precursor cell expressed, developmentally down-regulated 5, septin 2, septin-2
Entrez Gene IDs
4735 (Human)
Gene Symbol
SEPTIN2
UniProt
Additional Septin-2 Products
Product Documents for Septin-2 Antibody
Product Specific Notices for Septin-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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