Skip to main content

Septin-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20301

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-20301

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (99%), Porcine (98%), Rat (99%), Xenopus (94%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Septin-2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Septin-2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (94%).

Localization

Cytoplasm; Spindle

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Septin-2 Antibody

Western Blot: Septin-2 Antibody [NBP2-20301]

Western Blot: Septin-2 Antibody [NBP2-20301]

Western Blot: Septin-2 Antibody [NBP2-20301] - Sample (30 ug of whole cell lysate) A: U87-MG B: SK-N-SH 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP2-20301]

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP2-20301]

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP2-20301] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

Applications for Septin-2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Septin-2

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Alternate Names

DIFF6NEDD-5, hNedd5, KIAA0158Neural precursor cell expressed developmentally down-regulated protein 5, NEDD5Pnutl3, neural precursor cell expressed, developmentally down-regulated 5, septin 2, septin-2

Gene Symbol

SEPTIN2

UniProt

Additional Septin-2 Products

Product Documents for Septin-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Septin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.