Septin-5 Antibody (SP18) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81056

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Rat, Rabbit

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SP18

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Septin-5 Antibodies »

Product Specifications

Immunogen

Immunoprecipitate from human brain.

Specificity

This antibody recognises Septin-5 (CDCrel-1) and does not cross-react with CDCrel-2.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Septin-5 Antibody (SP18) - Chimeric - Azide and BSA Free

Western Blot: Septin-5 Antibody (SP18)ChimericAzide and BSA Free [NBP2-81056]

Western Blot: Septin-5 Antibody (SP18)ChimericAzide and BSA Free [NBP2-81056]

Western Blot: Septin-5 Antibody (SP18) [NBP2-81056] - Rat brain (lane 1), mouse spinal cord (lane 2), rat testis (lane 3), human lung cancer (lane 4) and human breast cancer (lane 5) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81056 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 43 kDa Septin-5 protein were detected in rat brain and mouse spinal chord. In this experiment no Septin-5 was detected in the other samples.
Immunohistochemistry: Septin-5 Antibody (SP18) - Chimeric - Azide and BSA Free [NBP2-81056]

Immunohistochemistry: Septin-5 Antibody (SP18) - Chimeric - Azide and BSA Free [NBP2-81056]

Immunohistochemistry: Septin-5 Antibody (SP18) [NBP2-81056] - Formalin fixed rabbit brain slices were were stained with NBP2-81056 at 3 ug/ml.

Applications for Septin-5 Antibody (SP18) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Septin-5

This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Two transcripts of this gene, a major one of 2.2 kb and a minor one of 3.5 kb, have been observed. The 2.2 kb form results from the utilization of a non-consensus polyA signal (AACAAT). In the absence of polyadenylation from this imperfect site, the consensus polyA signal of the downstream neighboring gene (GP1BB; platelet glycoprotein Ib) is used, resulting in the 3.5 kb transcript. An alternatively spliced transcript variant with a different 5' end has also been identified, but its full-length nature has not been completely determined. [provided by RefSeq]

Alternate Names

CDCREL, CDCREL1, CDCREL-1, cell division control related protein 1, Cell division control-related protein 1, HCDCREL-1, peanut-like 1, peanut-like 1 (Drosophila), Peanut-like protein 1, platelet glycoprotein Ib beta chain, PNUTL1H5, septin 5, septin-5

Gene Symbol

SEPTIN5

Additional Septin-5 Products

Product Documents for Septin-5 Antibody (SP18) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Septin-5 Antibody (SP18) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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