Septin-8 Antibody (JE64-99)

Western Blot: Septin-8 Antibody (JE64-99) [NBP3-32942]

Western Blot: Septin-8 Antibody (JE64-99) [NBP3-32942] - Western blot analysis of Septin-8 on different lysates with Rabbit anti-Septin-8 antibody (NBP3-32942) at 1/500 dilution.

Lane 1: K562 cell lysate
Lane 2: LO2 cell lysate
Lane 3: NIH/3T3 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 56 kDa
Observed band size: 54 kDa

Exposure time: 1 minute;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32942) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Septin-8 Antibody (JE64-99) [NBP3-32942]

Immunohistochemistry: Septin-8 Antibody (JE64-99) [NBP3-32942] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Septin-8 antibody (NBP3-32942) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32942) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.