SERCA1 ATPase Antibody (2T8S3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15830
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2T8S3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 900-1001 of human SERCA1 ATPase (O14983). ALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEDPEDERRK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SERCA1 ATPase Antibody (2T8S3)
![Western Blot: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830]](https://resources.bio-techne.com/images/products/SERCA1-ATPase-Antibody-2T8S3-Western-Blot-NBP3-15830-img0003.jpg "Western Blot: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830]")
Western Blot: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830]
Western Blot: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830] - Analysis of extracts of Mouse skeletal muscle, using SERCA1 ATPase Rabbit mAb (NBP3-15830) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830] -
Immunocytochemistry/ Immunofluorescence: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830] - Confocal imaging of paraffin-embedded Mouse skeletal muscle tissue using SERCA1 ATPase Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
Immunocytochemistry/ Immunofluorescence: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830] -
Immunocytochemistry/ Immunofluorescence: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830] - Confocal imaging of paraffin-embedded Rat skeletal muscle tissue using SERCA1 ATPase Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.Applications for SERCA1 ATPase Antibody (2T8S3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SERCA1 ATPase
Long Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
Alternate Names
ATP2A1, Calcium pump 1, EC 7.2.2.10, SERCA1, SR Ca(2+)-ATPase 1
Gene Symbol
ATP2A1
Additional SERCA1 ATPase Products
Product Documents for SERCA1 ATPase Antibody (2T8S3)
Product Specific Notices for SERCA1 ATPase Antibody (2T8S3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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