SERCA1 ATPase Antibody (2T8S3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15830

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15830-100ul
NBP3-15830-20ul
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2T8S3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SERCA1 ATPase Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 900-1001 of human SERCA1 ATPase (O14983). ALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEDPEDERRK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SERCA1 ATPase Antibody (2T8S3)

Western Blot: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830]

Western Blot: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830]

Western Blot: SERCA1 ATPase Antibody (2T8S3) [NBP3-15830] - Analysis of extracts of Mouse skeletal muscle, using SERCA1 ATPase Rabbit mAb (NBP3-15830) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for SERCA1 ATPase Antibody (2T8S3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SERCA1 ATPase

SERCA1 ATPase encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in two transcript variants encoding different isoforms.

Long Name

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Alternate Names

ATP2A1, Calcium pump 1, EC 7.2.2.10, SERCA1, SR Ca(2+)-ATPase 1

Gene Symbol

ATP2A1

Additional SERCA1 ATPase Products

Product Documents for SERCA1 ATPase Antibody (2T8S3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SERCA1 ATPase Antibody (2T8S3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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